Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Contents The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris‐glucose‐egg yolk extender (TE) was used for cool...

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Veröffentlicht in:Reproduction in domestic animals 2018-04, Vol.53 (2), p.525-533
Hauptverfasser: Pinyopummin, A, Mahasawangkul, S, Nunklang, G, Kornkaewrat, K, Laopiem, S, Koonjaenak, S, Wattananit, P
Format: Artikel
Sprache:eng
Schlagworte:
Acrosome - physiology
Animals
Asian elephant
Cell Survival
cooled storage
Cooling
Dilution
dilution effect
Elephants
Experiments
Horses
Integrity
Male
Males
Motility
Restoration
Semen
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Sperm
Sperm Motility - physiology
Spermatozoa - physiology
Storage
Viability
Yolk
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Zusammenfassung:Contents The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris‐glucose‐egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr. In experiment 1 (n = 18), semen was diluted to 1:1, 1:3, 1:7 and 1:15 with TE (volume per volume). There were no significant changes in sperm viability and sperm with normal acrosome integrity among dilutions, but sperm motility and motility velocities were greater (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13141