High-capacity ice-recrystallization endpoint assay employing superhydrophobic coatings that is equivalent to the ‘splat’ assay

We have developed an ice recrystallization inhibition (IRI) assay system that allows the side-by-side comparison of up to a dozen samples treated in an identical manner. This system is ideal for determining, by serial dilution, the IRI ‘endpoint’ where the concentration of a sample is reached that c...

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Veröffentlicht in:	Cryobiology 2018-04, Vol.81, p.138-144
Hauptverfasser:	Graham, Laurie A., Agrawal, Prashant, Oleschuk, Richard D., Davies, Peter L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antifreeze protein Antifreeze Proteins - chemistry Crystallization Endpoint Determination Freezing High-Throughput Screening Assays - methods Ice Ice recrystallization inhibition Phase Transition
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Beschreibung
Zusammenfassung:	We have developed an ice recrystallization inhibition (IRI) assay system that allows the side-by-side comparison of up to a dozen samples treated in an identical manner. This system is ideal for determining, by serial dilution, the IRI ‘endpoint’ where the concentration of a sample is reached that can no longer inhibit recrystallization. Samples can be an order of magnitude smaller in volume (
ISSN:	0011-2240 1090-2392
DOI:	10.1016/j.cryobiol.2018.01.011