Selected serum oxidative stress biomarkers in dogs with non‐food‐induced and food‐induced atopic dermatitis

Background Oxidative stress (OS) has been shown to be involved in the pathogenesis of human and canine atopic dermatitis (AD) through several distinct mechanisms. Selected serum biomarkers of OS (sbOS) have been validated in normal dogs and studied in several canine diseases. To the best of the authors’ knowledge, the sbOS evaluated in this study have not previously been described in canine AD. Hypothesis/Objectives The aims of the study were to evaluate a panel of sbOS in dogs with food‐induced (FIAD) and non‐food‐induced (NFIAD) AD: cupric reducing antioxidant capacity (CUPRAC), ferrous oxidation‐xylenol orange (FOX), ferric reducing ability of the plasma (FRAP), paraoxonase‐1 (PON1), trolox equivalent antioxidant capacity (TEAC) and serum total thiol (THIOL). The aim was to compare these metabolites with those in healthy control dogs, and to correlate sbOS with validated pruritus and CADESI‐04 severity scales in dogs with AD. Animals Forty six healthy, nine NFIAD and three FIAD client‐owned dogs were included. Methods The study was designed as a cohort study. Results There were significant differences in atopic dogs when compared to healthy dogs for all of the sbOS analysed. Conclusions and clinical relevance These findings suggest that OS could play a role in the pathogenesis of canine NFIAD and FIAD. In addition, the evaluation of sbOS could be useful for precision medicine to help to detect atopic dogs that might benefit from antioxidant‐targeted therapies. Résumé Contexte Le stress oxydatif (OS) a été rapporté comme étant impliqué dans la pathogénie de la dermatite atypique humaine et canine (AD) à travers plusieurs mécanismes distincts. Des biomarqueurs sériques sélectionnés (sbOS) ont été validés chez le chien normal et étudiés dans plusieurs pathologies canines. A la connaissance des auteurs, les sbOS évalués dans cette étude n'ont pas été précédemment décrit chez le chien atopique. Hypothèses/Objectifs Les buts de cette étude étaient d’évaluer un panel de sbOS chez les chiens atteints de AD liée à l'alimentation (FIAD) et de AD non liée à l'alimentation (NFIAD): CUPRAC (cupric reducing antioxidant capacity), FOX (ferrous oxidation‐xylenol orange), FRAP (ferric reducing ability of the plasma), PON1 (paraoxonase‐1), TEAC (trolox equivalent antioxidant capacity) et THIOL (serum total thiol). Sujets Quarante six chiens sains, neuf chiens NFIAD et trois chiens FIAD, de propriétaires ont été inclus. Méthodes Ceci est une étude de cohorte. Résultats Il