Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee

Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee

The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a...

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Veröffentlicht in:Journal of microbiological methods 2018-03, Vol.146, p.61-63
Hauptverfasser: Dainat, Benjamin, Grossar, Daniela, Ecoffey, Brigitte, Haldemann, Christoph
Format: Artikel
Sprache:eng
Schlagworte:
Apis mellifera
Diagnosis
Melissococcus plutonius
Multiplex real-time PCR
Paenibacillus larvae
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Beschreibung
Zusammenfassung:The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2018.01.018