Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes
Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreove...
Gespeichert in:
| Veröffentlicht in: | Marine environmental research 2004-08, Vol.58 (2), p.719-723 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 723 |
|---|---|
| container_issue | 2 |
| container_start_page | 719 |
| container_title | Marine environmental research |
| container_volume | 58 |
| creator | Regala, R.P. Rice, C.D. |
| description | Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass,
Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish,
Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen
Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl
2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to
M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure. |
| doi_str_mv | 10.1016/j.marenvres.2004.03.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19939529</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141113604001187</els_id><sourcerecordid>19939529</sourcerecordid><originalsourceid>FETCH-LOGICAL-c490t-ddd88eaddb7490b8e697b12e64abde0e9c25c1b18746e607e12b470eb24f0c213</originalsourceid><addsrcrecordid>eNqFkttuEzEQhlcIREPhFcA3IJCSMLPZky-ripZKjbgJ15YPE-Jo4w0-tOT5eDG8JOJwFcmSPdY3_2-P_qJ4gzBHwObjdr6TntyDpzAvAao5LOZQ4pNigl3LZ1ByfFpMACucIS6ai-JFCFsAqFusnxcXWGPbIVST4ufyoAcldSRv5ZSpFJkbItuR18kfpsw6kzSFfBFl3w9xYwdH1rH3y9UHtvdDHIu8QvR2T4YpGcKULQefMRbkDxttb_PNfiO_DfoQKZ8fN7YnprJYbrO71NuTDVuuRi29kc5Rz7SMaxs2U3ans3nyKbB9cvkc03-KL4tna9kHenXaL4uvN59W159n919u766v7me64hBnxpiuI2mManOtOmp4q7CkppLKEBDXZa1R5QFWDTXQEpaqaoFUWa1Bl7i4LN4ddfO_vycKUexs0NT30tGQgkDOF7wu-XmwgwaRw3kw-_OmHa3bI6j9EIKntdh7myNwEAhiTITYij-JEGMiBCwE_H7065NFUjsyf_tOEcjA2xMgg5b92kunbfiH6-oOm1Ho6shRHvGDJS-CtuQ0GetJR2EGe_YxvwAMg90m</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14709671</pqid></control><display><type>article</type><title>Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Regala, R.P. ; Rice, C.D.</creator><creatorcontrib>Regala, R.P. ; Rice, C.D.</creatorcontrib><description>Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass,
Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish,
Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen
Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl
2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to
M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure.</description><identifier>ISSN: 0141-1136</identifier><identifier>EISSN: 1879-0291</identifier><identifier>DOI: 10.1016/j.marenvres.2004.03.021</identifier><identifier>PMID: 15178104</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; Antibodies - immunology ; Bass - metabolism ; Bass - microbiology ; Biological and medical sciences ; Blotting, Western ; Catfishes - metabolism ; Catfishes - microbiology ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Ictalurus punctatus ; Inflammation ; Marine ; Mercury ; Mercury - toxicity ; Metallothionein ; Metallothionein - biosynthesis ; Metallothionein - genetics ; Metallothionein - immunology ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Morone ; Morone saxatilis ; Mycobacteria ; Mycobacterium ; Mycobacterium - pathogenicity ; Phagocytes - metabolism ; Sea water ecosystems ; Sequence Analysis, DNA ; Striped bass ; Synecology</subject><ispartof>Marine environmental research, 2004-08, Vol.58 (2), p.719-723</ispartof><rights>2004</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-ddd88eaddb7490b8e697b12e64abde0e9c25c1b18746e607e12b470eb24f0c213</citedby><cites>FETCH-LOGICAL-c490t-ddd88eaddb7490b8e697b12e64abde0e9c25c1b18746e607e12b470eb24f0c213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.marenvres.2004.03.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15858161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15178104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Regala, R.P.</creatorcontrib><creatorcontrib>Rice, C.D.</creatorcontrib><title>Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes</title><title>Marine environmental research</title><addtitle>Mar Environ Res</addtitle><description>Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass,
Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish,
Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen
Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl
2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to
M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure.</description><subject>Amino Acid Sequence</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Bass - metabolism</subject><subject>Bass - microbiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Catfishes - metabolism</subject><subject>Catfishes - microbiology</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Ictalurus punctatus</subject><subject>Inflammation</subject><subject>Marine</subject><subject>Mercury</subject><subject>Mercury - toxicity</subject><subject>Metallothionein</subject><subject>Metallothionein - biosynthesis</subject><subject>Metallothionein - genetics</subject><subject>Metallothionein - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Morone</subject><subject>Morone saxatilis</subject><subject>Mycobacteria</subject><subject>Mycobacterium</subject><subject>Mycobacterium - pathogenicity</subject><subject>Phagocytes - metabolism</subject><subject>Sea water ecosystems</subject><subject>Sequence Analysis, DNA</subject><subject>Striped bass</subject><subject>Synecology</subject><issn>0141-1136</issn><issn>1879-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkttuEzEQhlcIREPhFcA3IJCSMLPZky-ripZKjbgJ15YPE-Jo4w0-tOT5eDG8JOJwFcmSPdY3_2-P_qJ4gzBHwObjdr6TntyDpzAvAao5LOZQ4pNigl3LZ1ByfFpMACucIS6ai-JFCFsAqFusnxcXWGPbIVST4ufyoAcldSRv5ZSpFJkbItuR18kfpsw6kzSFfBFl3w9xYwdH1rH3y9UHtvdDHIu8QvR2T4YpGcKULQefMRbkDxttb_PNfiO_DfoQKZ8fN7YnprJYbrO71NuTDVuuRi29kc5Rz7SMaxs2U3ans3nyKbB9cvkc03-KL4tna9kHenXaL4uvN59W159n919u766v7me64hBnxpiuI2mManOtOmp4q7CkppLKEBDXZa1R5QFWDTXQEpaqaoFUWa1Bl7i4LN4ddfO_vycKUexs0NT30tGQgkDOF7wu-XmwgwaRw3kw-_OmHa3bI6j9EIKntdh7myNwEAhiTITYij-JEGMiBCwE_H7065NFUjsyf_tOEcjA2xMgg5b92kunbfiH6-oOm1Ho6shRHvGDJS-CtuQ0GetJR2EGe_YxvwAMg90m</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Regala, R.P.</creator><creator>Rice, C.D.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7QH</scope><scope>7QL</scope><scope>7TN</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>H96</scope></search><sort><creationdate>20040801</creationdate><title>Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes</title><author>Regala, R.P. ; Rice, C.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-ddd88eaddb7490b8e697b12e64abde0e9c25c1b18746e607e12b470eb24f0c213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Bass - metabolism</topic><topic>Bass - microbiology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Catfishes - metabolism</topic><topic>Catfishes - microbiology</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Ictalurus punctatus</topic><topic>Inflammation</topic><topic>Marine</topic><topic>Mercury</topic><topic>Mercury - toxicity</topic><topic>Metallothionein</topic><topic>Metallothionein - biosynthesis</topic><topic>Metallothionein - genetics</topic><topic>Metallothionein - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Morone</topic><topic>Morone saxatilis</topic><topic>Mycobacteria</topic><topic>Mycobacterium</topic><topic>Mycobacterium - pathogenicity</topic><topic>Phagocytes - metabolism</topic><topic>Sea water ecosystems</topic><topic>Sequence Analysis, DNA</topic><topic>Striped bass</topic><topic>Synecology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Regala, R.P.</creatorcontrib><creatorcontrib>Rice, C.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Regala, R.P.</au><au>Rice, C.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes</atitle><jtitle>Marine environmental research</jtitle><addtitle>Mar Environ Res</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>58</volume><issue>2</issue><spage>719</spage><epage>723</epage><pages>719-723</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass,
Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish,
Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen
Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl
2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to
M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15178104</pmid><doi>10.1016/j.marenvres.2004.03.021</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-1136 |
| ispartof | Marine environmental research, 2004-08, Vol.58 (2), p.719-723 |
| issn | 0141-1136 1879-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19939529 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence Animal and plant ecology Animal, plant and microbial ecology Animals Antibodies - immunology Bass - metabolism Bass - microbiology Biological and medical sciences Blotting, Western Catfishes - metabolism Catfishes - microbiology DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Ictalurus punctatus Inflammation Marine Mercury Mercury - toxicity Metallothionein Metallothionein - biosynthesis Metallothionein - genetics Metallothionein - immunology Mice Mice, Inbred BALB C Molecular Sequence Data Morone Morone saxatilis Mycobacteria Mycobacterium Mycobacterium - pathogenicity Phagocytes - metabolism Sea water ecosystems Sequence Analysis, DNA Striped bass Synecology |
| title | Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T20%3A54%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mycobacteria,%20but%20not%20mercury,%20induces%20metallothionein%20(MT)%20protein%20in%20striped%20bass,%20Morone%20saxitilis,%20phagocytes,%20while%20both%20stimuli%20induce%20MT%20in%20channel%20catfish,%20Ictalurus%20punctatus,%20phagocytes&rft.jtitle=Marine%20environmental%20research&rft.au=Regala,%20R.P.&rft.date=2004-08-01&rft.volume=58&rft.issue=2&rft.spage=719&rft.epage=723&rft.pages=719-723&rft.issn=0141-1136&rft.eissn=1879-0291&rft_id=info:doi/10.1016/j.marenvres.2004.03.021&rft_dat=%3Cproquest_cross%3E19939529%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14709671&rft_id=info:pmid/15178104&rft_els_id=S0141113604001187&rfr_iscdi=true |