Dairy Protein Supplementation Modulates the Human Skeletal Muscle microRNA Response to Lower Limb Immobilization

Limb immobilization results in a rapid loss of muscle size and strength. The resultant alterations in signaling pathways governing myogenesis, catabolism, and mitochondrial biogenesis are likely to include posttranscriptional regulation mediated by altered microRNAs (miRNAs). Given that protein ingestion exerts an anabolic action and may act as a countermeasure to mitigate muscle loss with immobilization, it is important to examine miRNA in this context. The objective of the study is therefore to characterize the vastus lateralis miRNA response to 14 days of disuse in males (45–60 years) randomized to receive supplementation with 20 g d−1 of dairy protein (n = 12) or isocaloric carbohydrate placebo (n = 13). Biopsies are collected before and after a 2‐week immobilization period. Of the 24 miRNAs previously identified in myogenic regulation, seven (miR‐133a, −206, −15a, −451a, −126, −208b, and let‐7e) are increased with immobilization irrespective of group; five (miR‐16, −494, let‐7a, −7c, and 7d) increased only in the carbohydrate group; and eight (miR‐1, −486, −23a, −23b, −26a, −148b, let‐7b, and −7g) are divergently expressed between groups (suppressed with protein). The ability of protein supplementation to differentially regulate miRNAs involved in key muscle regulatory pathways following short‐term limb immobilization reflects potential protective function in mitigating muscle loss during limb immobilization. Lower‐limb immobilization in middle‐aged men alters intramuscular microRNA profiles, reflecting signaling pathways regulate muscle fiber type, myogenesis, oxidative capacity, and apoptosis. Dairy protein supplementation attenuated disuse induces increases in 13 microRNAs. A 20 g dose of dairy protein ingested daily during disuse results in a microRNA profile that may reflect the maintenance of muscle metabolism.