The clinicopathological features and prognosis of tumor MSI in East Asian colorectal cancer patients using NCI panel

To compare test results obtained from a PCR assay for the National Cancer Institute (NCI) five loci criteria for detecting microsatellite instability (MSI) with those obtained from immunohistochemistry of mismatch repair and a five-mononucleotide site amplification system in East Asian patients with colorectal cancer. A total of 245 East Asian patients with colorectal cancer were studied retrospectively at our institution. The consistency of the NCI panel PCR method compared with detection of mismatch repair protein expression by immunohistochemistry was 0.898. High level MSI (MSI-H) status was correlated with the Tumor, Node, Metastasis stage, tumor location site, metastasis, tumor grade, mucinous histological type and BRAF-type mutations. The NCI panel PCR assay has excellent sensitivity and specificity for detecting MSI in an East Asian population.