Experimental study of influence of lipophilic products of phytogenic origin on lipid metabolism in rats

The article presents the results of biochemical evaluation of metabolic effects of lipophilic products of plant origin among which such oils as linseed, black nuts and walnuts oils as well as medicine «Phosphogliv» were selected as the most promising ones. The influence of the studied substances on lipid metabolism in experiment on male rats (170–220 g body weight) with modeled acute hepatotoxicity with carbon tetrachloride (that was achieved by subcutaneous injection of 50% oil solution of carbon tetrachloride – 0.5 ml/100 g of the body mass once a day during 3 days) has been investigated. Liver function was assessed by triacylglycerols content in the serum, total, esterified and nonesterified cholesterol, cholesterol in the lipoproteins of high, low and very low density, as well as by the nonesterified cholesterol and phospholipids content in the hemolysate of red blood cells. Carbon tetrachloride hepatotoxic damage was accompanied by the development of severe hypercholesterolemia associated both with the increase in total cholesterol and its content in low density lipoproteins alongside the reducing of the cholesterol concentration in high density lipoproteins, resulted in secondary dyslipoproteinemia. Inhibition of the esterification of cholesterol processes as well as the decrease in the triacylglycerols concentration was observed. It is connected with the triacylglycerols endogenous synthesis blocking in the liver, resulted from its toxic damage. It is also confirmed by cholesterol content reducing in the lipoproteins of very low density. In erythrocytes of rats with CCl4 intoxication phospholipid content decreased while the amount of nonesterified cholesterol that is a component of cell membranes, influencing the proteins and lipids diffusion, which reduces the mobility of the fatty acid residues of phospholipids, increased. The injection of the black nuts and walnuts oils as well as flax oil (intragastric injections 0.2 ml daily in the morning before the main feeding from the 7th to the 30th day of the experiment, n=25 in each group) to rats with liver failure induced by carbon tetrachloride, contributed to the partial restoration of liver tissue structure and statistically reliable decrease of lipid metabolism. Decrease in the total cholesterol content by 17.5% in the group of animals treated with linseed oil was observed; LDL cholesterol also decreased under the influence of walnuts and black nuts oils by 36.7 and 40.6% respectively. The increase