Rapid detection of blaKPC directly from surveillance rectal swabs by EasyQ KPC

The performance of EasyQ KPC assay was evaluated for the first time for blaKPC detection directly from surveillance rectal swabs without broth enrichment. Using conventional polymerase chain reaction as gold standard method, EasyQ KPC and culture-based molecular tests demonstrated a sensitivity/spec...

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Veröffentlicht in:Diagnostic microbiology and infectious disease 2018-04, Vol.90 (4), p.251-252
Hauptverfasser: Ramos, Ana Carolina, Schandert, Lygia, Carvalhaes, Cecilia G., Martins, Rafael Affini, Cappellano, Paola, Machado, Flávia Ribeiro, Gales, Ana Cristina
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Sprache:eng
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Zusammenfassung:The performance of EasyQ KPC assay was evaluated for the first time for blaKPC detection directly from surveillance rectal swabs without broth enrichment. Using conventional polymerase chain reaction as gold standard method, EasyQ KPC and culture-based molecular tests demonstrated a sensitivity/specificity of 100%/87.3% and 83.3%/98.2%, respectively. •Three different methods for the detection of blaKPC from rectal swabs were performed.•Detection of blaKPC was evaluated directly from rectal swabs without broth enrichment.•EasyQ KPC demonstrated to be a rapid and feasible assay for the detection of blaKPC.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2017.12.009