Correlation between miR-19a inhibition and radiosensitivity in SiHa cervical cancer cells
MicroRNAs (miRs) have been implicated in many aspects of tumor cell development and survival, including sensitivity to radiotherapy. In particular, miR-19a regulates the proliferation of cervical cancer cells, but its role in radiosensitivity is not known. Here, we describe the consequence of silenc...
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Veröffentlicht in: | Journal of B.U. ON. 2017-11, Vol.22 (6), p.1505-1508 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | MicroRNAs (miRs) have been implicated in many aspects of tumor cell development and survival, including sensitivity to radiotherapy. In particular, miR-19a regulates the proliferation of cervical cancer cells, but its role in radiosensitivity is not known. Here, we describe the consequence of silencing miR-19a using antisense oligonucleotides (ASO) on the radiosensitivity of human cervical cancer SiHa cells.
Following transfection with miR-19a-ASO or control-ASO, SiHa cells were exposed to X-rays to determine their proliferation.
Silencing of miR-19a significantly improved the sensitivity of SiHa cells to radiotherapy by reducing proliferation, increasing apoptosis, upregulating BAX, and downregulating Bcl-2.
Overall, inhibiting miR-19a significantly improves the sensitivity of SiHa cells to radiotherapy, which could lead to new methods for the treatment of cervical cancer. |
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ISSN: | 1107-0625 |