Correlation between miR-19a inhibition and radiosensitivity in SiHa cervical cancer cells

MicroRNAs (miRs) have been implicated in many aspects of tumor cell development and survival, including sensitivity to radiotherapy. In particular, miR-19a regulates the proliferation of cervical cancer cells, but its role in radiosensitivity is not known. Here, we describe the consequence of silenc...

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Veröffentlicht in:Journal of B.U. ON. 2017-11, Vol.22 (6), p.1505-1508
Hauptverfasser: Wang, Yina, Wang, Yang, Zhong, Wei, Gulina, Kuerban
Format: Artikel
Sprache:eng
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Zusammenfassung:MicroRNAs (miRs) have been implicated in many aspects of tumor cell development and survival, including sensitivity to radiotherapy. In particular, miR-19a regulates the proliferation of cervical cancer cells, but its role in radiosensitivity is not known. Here, we describe the consequence of silencing miR-19a using antisense oligonucleotides (ASO) on the radiosensitivity of human cervical cancer SiHa cells. Following transfection with miR-19a-ASO or control-ASO, SiHa cells were exposed to X-rays to determine their proliferation. Silencing of miR-19a significantly improved the sensitivity of SiHa cells to radiotherapy by reducing proliferation, increasing apoptosis, upregulating BAX, and downregulating Bcl-2. Overall, inhibiting miR-19a significantly improves the sensitivity of SiHa cells to radiotherapy, which could lead to new methods for the treatment of cervical cancer.
ISSN:1107-0625