A molecular mechanics study on GA codon box translation

•AMBER molecular mechanics study.•Codon-anticodon duplexes involving GA codon box.•Twofold degeneracy of aspartic acid and glutamic acid.•Distinguish between cognate and non-cognate interactions on the basis of duplex stability and structure.•Non-cognate duplexes involving alanine anticodons found to be distorted. The GA codon box incorporates the two-fold degeneracy of aspartic acid and of glutamic acid. Using the molecular mechanics approach of the AMBER suite, the four codons of the GA box are paired via H-bonding with two aspartic acid anticodons and two glutamic acid anticodons to yield 8 cognate and 11 non-cognate codon-anticodon duplexes. In addition four select non-cognate duplexes between the GA box codons and three alanine anticodons are also studied. These 23 duplexes display a variety of base-pairing possibilities at the wobble position. Cognate duplexes are differentiated from non-cognate duplexes on the grounds of structure and stability (chiefly the former). The results are in line with Crick's wobble hypothesis, and corroborate the observed reading properties of the aspartic acid anticodons GUC and QUC and of the glutamic acid anticodons CUC and SmnUC.