Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area
The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass,...
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| Veröffentlicht in: | Dental materials 2018-03, Vol.34 (3), p.452-459 |
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| creator | Turco, Gianluca Cadenaro, Milena Maravić, Tatjana Frassetto, Andrea Marsich, Eleonora Mazzoni, Annalisa Di Lenarda, Roberto Tay, Franklin R. Pashley, David H. Breschi, Lorenzo |
| description | The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area.
Non-carious human molars (N=24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37mm, 0.75mm, and 1.50mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit.
A statistically significant (p |
| doi_str_mv | 10.1016/j.dental.2017.12.003 |
| format | Article |
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Non-carious human molars (N=24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37mm, 0.75mm, and 1.50mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit.
A statistically significant (p<0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p<0.05).
Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.</description><identifier>ISSN: 0109-5641</identifier><identifier>EISSN: 1879-0097</identifier><identifier>DOI: 10.1016/j.dental.2017.12.003</identifier><identifier>PMID: 29301651</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Aging ; Cathepsins ; Collagen ; Collagen degradation ; Collagen Type I - metabolism ; CTX ; Demineralizing ; Dentin ; Dentin - metabolism ; Dentistry ; Enzyme-Linked Immunosorbent Assay ; Fragmentation ; Fragments ; Gelatinase A ; Humans ; ICTP ; Impact analysis ; In Vitro Techniques ; Matrix metalloproteinases ; Matrix Metalloproteinases - metabolism ; Molar ; Molars ; Molecular chains ; Peptides - metabolism ; Saliva ; Saliva, Artificial ; Slabs ; Statistical analysis ; Substrates ; Surface area ; Teeth ; Time ; Time dependence ; Time Factors</subject><ispartof>Dental materials, 2018-03, Vol.34 (3), p.452-459</ispartof><rights>2017 The Academy of Dental Materials</rights><rights>Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Mar 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-386e9a5d1ecec5d5eb40f24b103e27c5348b091873a2a3b419b97b52699ba3503</citedby><cites>FETCH-LOGICAL-c390t-386e9a5d1ecec5d5eb40f24b103e27c5348b091873a2a3b419b97b52699ba3503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dental.2017.12.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29301651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turco, Gianluca</creatorcontrib><creatorcontrib>Cadenaro, Milena</creatorcontrib><creatorcontrib>Maravić, Tatjana</creatorcontrib><creatorcontrib>Frassetto, Andrea</creatorcontrib><creatorcontrib>Marsich, Eleonora</creatorcontrib><creatorcontrib>Mazzoni, Annalisa</creatorcontrib><creatorcontrib>Di Lenarda, Roberto</creatorcontrib><creatorcontrib>Tay, Franklin R.</creatorcontrib><creatorcontrib>Pashley, David H.</creatorcontrib><creatorcontrib>Breschi, Lorenzo</creatorcontrib><title>Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area</title><title>Dental materials</title><addtitle>Dent Mater</addtitle><description>The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area.
Non-carious human molars (N=24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37mm, 0.75mm, and 1.50mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit.
A statistically significant (p<0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p<0.05).
Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.</description><subject>Aging</subject><subject>Cathepsins</subject><subject>Collagen</subject><subject>Collagen degradation</subject><subject>Collagen Type I - metabolism</subject><subject>CTX</subject><subject>Demineralizing</subject><subject>Dentin</subject><subject>Dentin - metabolism</subject><subject>Dentistry</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fragmentation</subject><subject>Fragments</subject><subject>Gelatinase A</subject><subject>Humans</subject><subject>ICTP</subject><subject>Impact analysis</subject><subject>In Vitro Techniques</subject><subject>Matrix metalloproteinases</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Molar</subject><subject>Molars</subject><subject>Molecular chains</subject><subject>Peptides - metabolism</subject><subject>Saliva</subject><subject>Saliva, Artificial</subject><subject>Slabs</subject><subject>Statistical analysis</subject><subject>Substrates</subject><subject>Surface area</subject><subject>Teeth</subject><subject>Time</subject><subject>Time dependence</subject><subject>Time Factors</subject><issn>0109-5641</issn><issn>1879-0097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1r3DAQhkVpaTZp_0EJgl56iN0ZyVpbORTKkqSBQEvZQm9ClsagxR8byQ60v77abpJDDz3NQc88M5qXsXcIJQKuP-5KT-Ns-1IA1iWKEkC-YCtsal0A6PolWwGCLtS6whN2mtIOACqh8TU7EVpmhcIVG79TTzYRnzp-u9l-43b0fLP9yWfqpz3t5-Ap8S5OA_c0hJGi7cNv8vwwPIx8sHMMjtIlv-o6cvPBM4eBLvJLSn9taYmddcRtJPuGvepsn-jtYz1jP66vtpsvxd3Xm9vN57vCSQ1zIZs1aas8kiOnvKK2gk5ULYIkUTslq6YFnb8qrbCyrVC3um6VWGvdWqlAnrEPR-8-TvcLpdkMITnqezvStCSDutFK1gKajL7_B91NSxzzdkZAhbWSqtGZqo6Ui1NKkTqzj2Gw8ZdBMIc8zM4c8zCHPAwKk_PIbeeP8qUdyD83PQWQgU9HgPI1HgJFk1yg0ZEPMZ_T-Cn8f8If84CcBA</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Turco, Gianluca</creator><creator>Cadenaro, Milena</creator><creator>Maravić, Tatjana</creator><creator>Frassetto, Andrea</creator><creator>Marsich, Eleonora</creator><creator>Mazzoni, Annalisa</creator><creator>Di Lenarda, Roberto</creator><creator>Tay, Franklin R.</creator><creator>Pashley, David H.</creator><creator>Breschi, Lorenzo</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201803</creationdate><title>Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area</title><author>Turco, Gianluca ; 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The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area.
Non-carious human molars (N=24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37mm, 0.75mm, and 1.50mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit.
A statistically significant (p<0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p<0.05).
Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>29301651</pmid><doi>10.1016/j.dental.2017.12.003</doi><tpages>8</tpages></addata></record> |
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| subjects | Aging Cathepsins Collagen Collagen degradation Collagen Type I - metabolism CTX Demineralizing Dentin Dentin - metabolism Dentistry Enzyme-Linked Immunosorbent Assay Fragmentation Fragments Gelatinase A Humans ICTP Impact analysis In Vitro Techniques Matrix metalloproteinases Matrix Metalloproteinases - metabolism Molar Molars Molecular chains Peptides - metabolism Saliva Saliva, Artificial Slabs Statistical analysis Substrates Surface area Teeth Time Time dependence Time Factors |
| title | Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area |
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