Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area

Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area

The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass,...

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Veröffentlicht in:Dental materials 2018-03, Vol.34 (3), p.452-459
Hauptverfasser: Turco, Gianluca, Cadenaro, Milena, Maravić, Tatjana, Frassetto, Andrea, Marsich, Eleonora, Mazzoni, Annalisa, Di Lenarda, Roberto, Tay, Franklin R., Pashley, David H., Breschi, Lorenzo
Format: Artikel
Sprache:eng
Schlagworte:
Aging
Cathepsins
Collagen
Collagen degradation
Collagen Type I - metabolism
CTX
Demineralizing
Dentin
Dentin - metabolism
Dentistry
Enzyme-Linked Immunosorbent Assay
Fragmentation
Fragments
Gelatinase A
Humans
ICTP
Impact analysis
In Vitro Techniques
Matrix metalloproteinases
Matrix Metalloproteinases - metabolism
Molar
Molars
Molecular chains
Peptides - metabolism
Saliva
Saliva, Artificial
Slabs
Statistical analysis
Substrates
Surface area
Teeth
Time
Time dependence
Time Factors
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Zusammenfassung:The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area. Non-carious human molars (N=24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37mm, 0.75mm, and 1.50mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit. A statistically significant (p
ISSN:0109-5641
1879-0097
DOI:10.1016/j.dental.2017.12.003