Structural analysis of the O-polysaccharide from the lipopolysaccharide of Pseudomonas putida BIM B-1100

Two specific polysaccharides, together with an →4)-α-d-Glcp-(1→ glucan (bacterial glycogen), were obtained from a lipopolysaccharide preparation isolated from the bacterium Pseudomonas putida BIM B-1100 by phenol/water extraction. The following structures of the polysaccharides were established by composition analysis, Smith degradation, ESI-MS, and 1D and 2D NMR spectroscopy: [Display omitted] [Display omitted] •Lipopolysaccharide was isolated from Pseudomonas putida BIM B-1100.•Two O-polysaccharides were obtained by mild acid degradation of the lipopolysaccharide.•The O-polysaccharides were partially O-acetylated.•Structures of the O-polysaccharides were established by chemical methods and NMR spectroscopy.