Analysis of Bovine Growth Hormone Gene Polymorphisms in Three Iranian Native Breeds and Holstein Cattle by RFLP-PCR

The aim of this study was to estimate the allelic frequency in polymorphic sites of intron 3 and exon 5 of bovine growth hormone gene in three Iranian native and Holstein cattle. A total of 406 genomic DNA samples were extracted from three Iranian native cattle including, Mazandarani 97, Sarabi 87, Golpaygani 112 and Holstein 110 cattle. The PCR procedure was used to amplify 345 bp of bGH-intron 3 and 404 bp of bGH-exon 5. The frequencies of MspI (+) allele were estimated as 0.55, 0.51, 0.44 and 0.83 in Mazandarani, Sarabi, Golpaygani and Holstein breed, respectively. The allelic frequencies of AluI (+) were calculated as 0.91, 0.84, 0.92, 0.85 and for DdeI (+), 0.52, 0.54, 0.47 and 0.86, for these breeds, respectively. Chi-square test showed significant differences (p