Antiviral Activity of Euphorbia helioscopia Extract
In the present study, the antiviral effects of Euphorbia helioscopia extracts were investigated using plaque reduction assay. Plant extracts were prepared using Soxhlet apparatus or by maceration in methanol. After applying several enriching stages of phage CP51, phage titration was performed to det...
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Veröffentlicht in: | Journal of biological sciences (Faisalabad, Pakistan) Pakistan), 2008-05, Vol.8 (4), p.809-813 |
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Sprache: | eng |
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Zusammenfassung: | In the present study, the antiviral effects of Euphorbia helioscopia extracts were investigated using plaque reduction assay. Plant extracts were prepared using Soxhlet apparatus or by maceration in methanol. After applying several enriching stages of phage CP51, phage titration was performed to determine the phage concentration in phage lysate for specifying the dilution factor of the phage to be used as negative control for the next working stages. Then IC sub(50) of trifluridine, as a positive control, for phage CP51 was determined. The MIC of the extracts for Bacillus cereus was determined as 1.25 and 0.5 mg mL super(-1) for Soxhlet and maceration extracts, respectively. To determine whether the extracts have the ability to inhibit the adsorption of virus to host cell, it was pre-incubated with phage CP51 for 30 min at 25 degree C. The growth and reproduction of phage was inhibited by more than 50% at concentration of 1 and 0.25 mg mL super(-1), respectively. In order to test the effects of extract on transcription process, Bacillus cereus, phage CP51 and extract were incubated together. The growth and reproduction of phage was inhibited by more than 50% at concentration of 0.75 and 0.125 mg mL super(-1) or Soxhlet and macerated extracts, respectively. These results indicated that both extracts of E. helioscopia have considerable antiviral activity. |
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ISSN: | 1727-3048 |
DOI: | 10.3923/jbs.2008.809.813 |