KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4- lacZ-based cadmium and copper biosensor

KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4- lacZ-based cadmium and copper biosensor

Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a sh...

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Veröffentlicht in:Enzyme and microbial technology 2006-12, Vol.40 (1), p.67-70
Hauptverfasser: Freire-Picos, M.A., Lamas-Maceiras, M.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biosensor
Biotechnology
Cadmium
Copper
Fundamental and applied biological sciences. Psychology
Kluyveromyces lactis
Saccharomyces cerevisiae
Yeast
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Zusammenfassung:Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a shorter promoter not regulated by metals. This eukaryotic system can be used to detect cadmium and copper concentrations of 0.3–3 mM (or higher) within minutes using X-GAL filter assay and other variants of β-galactosidase assays developed in multititer wells.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2005.12.023