Development of bi-enzyme microbiosensor based on solid-contact ion-selective microelectrodes for protein detection

A novel electrochemical microbiosensor for protein determination based on an all-solid-state microelectrodes with a Ppy [3,3-Co(1,2-C 2B 9H 11) 2] solid internal contact and immobilized trypsin and carboxypeptidase B was developed. The specificity of the sensor signal was investigated using artificial ester and amide substrates of the proteinases: Nα-benzoyl- l-arginine ethyl ester hydrochloride (BAEE), Nα-benzoyl- dl-arginine- p-nitroanilide hydrochloride (BAPNA), and Hippuryl-Arg. Study on a possibility of protein detection showed that quantitative proteins determination can be performed with the microbiosensor in the concentration range of 1–60 μg/ml.