Rapid Whole Genome Amplification of DNA from Felids: Applications for Conservation Genetics

A major hindrance to conservation genetic studies of felids is the acquisition of DNA samples required for examining natural populations. In many cases research is complicated further by restrictions associated with the transport of biological materials from legally collected specimens. In this pape...

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Veröffentlicht in:Wildlife Society bulletin 2006-11, Vol.34 (4), p.1134-1141
Hauptverfasser: JANEČKA, JAN E, GRASSMAN, LON I, DERR, JAMES N, HONEYCUTT, RODNEY L, EIADTHONG, WICHAN, TEWES, MICHAEL E
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container_end_page 1141
container_issue 4
container_start_page 1134
container_title Wildlife Society bulletin
container_volume 34
creator JANEČKA, JAN E
GRASSMAN, LON I
DERR, JAMES N
HONEYCUTT, RODNEY L
EIADTHONG, WICHAN
TEWES, MICHAEL E
description A major hindrance to conservation genetic studies of felids is the acquisition of DNA samples required for examining natural populations. In many cases research is complicated further by restrictions associated with the transport of biological materials from legally collected specimens. In this paper we assessed a method of rapidly amplifying whole genomes, both nuclear and mitochondrial, with the use of a strand displacement reaction (SDR) and the Phi29 DNA polymerase enzyme. We evaluated the SDR product with downstream amplification of microsatellite loci for genetic analyses of felids. The amplicon was of high molecular weight and provided a template equivalent to original DNA for polymerase chain reaction amplification of 10 microsatellites when properly stored samples were used. Genotypes of 6 felid species derived from genome amplifications scored identical to the original DNA, and we observed only 1 allelic dropout event among 67 heterozygotes. The Phi29 enzyme was able to amplify the genomes in samples up to 19 years old and those with degraded DNA. We extracted DNA and SDR amplified the genomes of 48 individuals of 5 felid species in Thailand in
doi_str_mv 10.2193/0091-7648(2006)34[1134:RWGAOD]2.0.CO;2
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source Jstor Complete Legacy; Wiley Online Library Journals Frontfile Complete; BioOne Complete
subjects Alleles
Animal genetics
Animal populations
carnivore
Carnivore Research
Cats
Conservation biology
conservation genetics
DNA
DNA polymerase
Endangered & extinct species
felid
Genetic research
genome amplification
Genomes
Genomics
GenomiPhi
GenomiPhiTM
Medical genetics
Microsatellites
mitochondrial DNA
Molecular weight
Phi29 polymerase
Polymerase chain reaction
Temperature
Thailand
Tissues
Wildlife conservation
title Rapid Whole Genome Amplification of DNA from Felids: Applications for Conservation Genetics
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