High growth potential and nitrogen removal performance of marine anammox bacteria in shrimp-aquaculture sediment
Anaerobic ammonium oxidation (anammox) bacteria were enriched in continuous packed-bed columns with marine sediment. One column (SB-C) was packed with only marine sediment collected from a shrimp-aquaculture pond, and another column (SB-AMX) was inoculated with marine anammox bacteria (MAB) as a con...
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Veröffentlicht in: | Chemosphere (Oxford) 2018-04, Vol.196, p.69-77 |
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Sprache: | eng |
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Zusammenfassung: | Anaerobic ammonium oxidation (anammox) bacteria were enriched in continuous packed-bed columns with marine sediment. One column (SB-C) was packed with only marine sediment collected from a shrimp-aquaculture pond, and another column (SB-AMX) was inoculated with marine anammox bacteria (MAB) as a control. These columns were continuously fed with natural or artificial seawater including ammonium (NH4+) and nitrite (NO2−). The SB-AMX showed anammox activities from the beginning and continued for over 200 days. However, the SB-C had no nitrogen removal performance for over 170 days. After adding a bicarbonate solution (KHCO3) to the sediment-only packed column, anammox activity was observed within 13 days. The column exhibited a nitrogen removal efficiency (NRE) of 88% at a nitrogen loading rate (NLR) of 1.0 kg-N·m−3·day−1, which was comparable to the control one. A next-generation sequencing analysis revealed the predominance of MAB related to “Candidatus Scalindua spp.”. In addition, the co-occurrence of sulfur-oxidizing denitrifiers was observed, which suggests their symbiotic relationship. This study suggests the applicability of MAB for in-situ bioremediation of nitrogen-contaminated marine sediments and reveals a potential microbial interaction between anammox and sulfur-oxidizing communities responsible for nitrogen and sulfur cycling in marine aquaculture systems.
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•A cultivation system for anammox bacteria based on sediment was established.•Bicarbonate supplements effectively stimulated indigenous anammox bacteria.•Approximately 90% of nitrogen was removed at a high loading rate.•Coexistence with sulfur-oxidizing bacteria benefited anammox bacteria. |
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ISSN: | 0045-6535 1879-1298 |
DOI: | 10.1016/j.chemosphere.2017.12.159 |