A rapid method for protein extraction from fission yeast

A rapid method for protein extraction from fission yeast

Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein ex...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-08, Vol.70 (8), p.1992-1994
Hauptverfasser: Matsuo, Y.(Saga Univ. (Japan)), Asakawa, K, Toda, T, Katayama, S
Format: Artikel
Sprache:eng
Schlagworte:
ALKALI TREATMENT
alkaline extraction
BIOCHEMICAL ENGINEERING
Biological and medical sciences
EXTRACCION POR DISOLVENTES
EXTRACTION PAR SOLVANT
fission yeast
Fundamental and applied biological sciences. Psychology
GENIE BIOCHIMIQUE
IMMUNOBLOTTING
INGENIERIA BIOQUIMICA
LEVADURA
LEVURE
MICROBIAL PROTEINS
PROTEINAS MICROBIANAS
PROTEINE MICROBIENNE
Proteins - chemistry
Proteins - isolation & purification
Schizosaccharomyces - chemistry
Schizosaccharomyces - cytology
Schizosaccharomyces pombe
Sodium Hydroxide - chemistry
SOLVENT EXTRACTION
Time Factors
TRAITEMENT AUX ALCALIS
TRATAMIENTO ALCALINO
western blotting
YEASTS
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Beschreibung
Zusammenfassung:Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60087