Small Molecule Pin1 Inhibitor Blocking NF‐κB Signaling in Prostate Cancer Cells

Prolyl‐isomerase 1 (Pin1) is a conserved enzyme that regulates cell processes such as cell cycle progression, transcriptional regulation, and apoptosis. However, overexpression of Pin1 is correlated with a higher probability of prostate tumor recurrence. We utilized a molecular docking technique to identify Pin1 inhibitors from a database of natural product and natural product‐like compounds. The action of the hit compounds against Pin1 activity was studied using multiple methods, including a fluorometric enzymatic assay, co‐immunoprecipitation, western blotting, cell thermal shiftm, and other techniques. We have identified compound 1 as a natural‐product‐like inhibitor of Pin1 activity via structure‐based virtual screening and showed that compound 1 could target Pin1 and disrupt the interaction between Pin1 and the p65 subunit of NF‐κB in cells. Furthermore, compound 1 reduced nuclear p65 (Thr254) phosphorylation and attenuated NF‐κB activity in cells. Finally, compound 1 induced apoptosis in prostate cancer cells. Compound 1 represents a natural product‐like Pin1 inhibitor that acts via targeting the Pin1–NF‐κB interaction. Pinned down: The compound shown is a first‐class natural‐product‐like Pin1 inhibitor that acts via targeting the Pin1–p65 interaction.