Effect of l‐arginine addition on long‐term storability of ram semen

Summary This study was conducted to investigate the effect of l‐arginine addition on long‐term storability of ram semen. Six Akkaraman rams were used as material. Semen samples were collected. Pooled samples were diluted and were divided into six equal aliquots. While aliquot 1 was kept as control, the stock solutions including 0.1, 0.5, 1, 5 and 10 mm l‐arginine were added to other aliquots. All aliquots were routinely frozen in 0.25‐ml straws at −130°C liquid nitrogen vapour and stored in liquid nitrogen −196°C until being analysed. The equilibrated and thawed sperm motility, membrane integrity and arginase activity were evaluated. While the 10 mm l‐arginine supplementation significantly (p