Isolation and Characterization of the Shiga Toxin Gene (stx)-Bearing Escherichia coli O157 and Non-O157 from Retail Meats in Shandong Province, China, and Characterization of the O157-Derived stx sub(2) Phages

Infection by Shiga toxin (Stx)-producing Escherichia coli of non-O157 and O157 serotypes are rare in China, but infection by O157 serotype was found in Shandong Province and three other provinces in China. To understand the reason for these rare infections and to determine the safety of retail meats in Shandong Province, we examined the distribution of Shiga toxin gene (stx)-bearing E. coli in retail meats and characterized the isolated stx-bearing strains. We used hybridization with DNA probes and isolated stx sub(1)- and/or stx sub(2)-positive E. coli from 31 (58%) of 53 retail meat samples, with beef showing the highest frequency (68%). Of 42 stx-positive isolates, none belonged to O157. Using the O157-specific immunomagnetic bead technique, we isolated E. coli O157 carrying the eae and stx sub(2) genes from eight beef samples (26%). These strains produced little or no Stx2 and carried a unique q gene. Replication of the stx sub(2) phages was detected in these strains, whereas stx sub(2) phage replication was not detected in our previous study in which we examined similar stx sub(2)-bearing E. coli O157 strains from other Asian countries. Analysis of E. coli C600 lysogenized with the stx sub(2) phages found in this study suggests that the lack of Stx2 production is due to changes in non-q gene region(s) of the phage genome or chromosomal mutation(s) in the host. Our data and reports by other workers suggest it is necessary to determine if various stx sub(2)-bearing E. coli O157 strains producing Stx2 to varying degrees are distributed in meats in various locations in China.