Uridine 5′-polyphosphates (p 4U and p 5U) and uridine(5′)polyphospho(5′)nucleosides (Up nNs) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae

UTP:glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) from Saccharomyces cerevisiae can transfer the uridylyl moiety from UDP-glucose onto tripolyphosphate (P 3), tetrapolyphosphate (P 4), nucleoside triphosphates (p 3Ns) and nucleoside 5′-polyphosphates (p 4Ns) forming uridine 5′-tetraphosphate (p 4U), uridine 5′-pentaphosphate (p 5U) and dinucleotides, such as Ap 4U, Cp 4U, Gp 4U, Up 4U, Ap 5U and Gp 5U. Unlike UDP-glucose, UDP-galactose was not a UMP donor and ADP was not a UMP acceptor. This is the first example of an enzyme that may be responsible for accumulation of dinucleoside tetraphosphates containing two pyrimidine nucleosides in vivo. Occurrence of such dinucleotides in S. cerevisiae and Escherichia coli has been previously reported (Coste et al., J. Biol. Chem. 262 (1987) 12096–12103).