Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition
Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the bes...
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| Veröffentlicht in: | Journal of microbiological methods 2018-02, Vol.145, p.10-13 |
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| container_title | Journal of microbiological methods |
| container_volume | 145 |
| creator | Castaño, Jesus D. Zhang, Jiwei Schilling, Jonathan S. |
| description | Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.
•Hydrogen peroxide (H2O2) is a key oxidant produced by fungi decomposing wood.•Low H202 levels in decaying wood (in planta) evade detection via standard methods.•We compared H2O2 quantification in wood being decayed by a fungus Postia placenta.•In order of most sensitive to least Amplex Red10 |
| doi_str_mv | 10.1016/j.mimet.2017.12.004 |
| format | Article |
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•Hydrogen peroxide (H2O2) is a key oxidant produced by fungi decomposing wood.•Low H202 levels in decaying wood (in planta) evade detection via standard methods.•We compared H2O2 quantification in wood being decayed by a fungus Postia placenta.•In order of most sensitive to least Amplex Red10<ABTS<FOX<4-aminoantipyrine.•Amplex Red (LOQ=0.36μM) was best for measuring H2O2 (0.41–1.77μM) in planta.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2017.12.004</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Amplex red 10 ; Brown rot ; Fungi ; Radical</subject><ispartof>Journal of microbiological methods, 2018-02, Vol.145, p.10-13</ispartof><rights>2017 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2017.12.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Castaño, Jesus D.</creatorcontrib><creatorcontrib>Zhang, Jiwei</creatorcontrib><creatorcontrib>Schilling, Jonathan S.</creatorcontrib><title>Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition</title><title>Journal of microbiological methods</title><description>Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.
•Hydrogen peroxide (H2O2) is a key oxidant produced by fungi decomposing wood.•Low H202 levels in decaying wood (in planta) evade detection via standard methods.•We compared H2O2 quantification in wood being decayed by a fungus Postia placenta.•In order of most sensitive to least Amplex Red10<ABTS<FOX<4-aminoantipyrine.•Amplex Red (LOQ=0.36μM) was best for measuring H2O2 (0.41–1.77μM) in planta.</description><subject>Amplex red 10</subject><subject>Brown rot</subject><subject>Fungi</subject><subject>Radical</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kD1PwzAURS0EEqXwC1g8siT4I7GdgQFVhSJV6tLdcuyXylUSFzsp4t-TUMT0lnOv7jsIPVKSU0LF8zHvfAdDzgiVOWU5IcUVWlAlWaZ4WV2jxUTJTBLKbtFdSkdCaMkLtUCwPpt2NIMPPQ4NtqENca6K3mKTkvlOuAkROxggdr7_Bzdsx3yPT63pB4PdGH1_wM3YH0yLv0JwU8KG7hSSnxP36KYxbYKHv7tE-7f1frXJtrv3j9XrNgMlRVY21hrhRC1q20ioiSqMKkUF1FiQhZVQloQbVlguBHGccFpDZVRDS8qJYnyJni61pxg-R0iD7nyy0E4jIYxJ00pKqabfxYS-XFCY5pw9RJ2sh96C8xHsoF3wmhI929VH_WtXz3Y1ZXqyy38Aqn9xqA</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Castaño, Jesus D.</creator><creator>Zhang, Jiwei</creator><creator>Schilling, Jonathan S.</creator><general>Elsevier B.V</general><scope>7X8</scope></search><sort><creationdate>201802</creationdate><title>Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition</title><author>Castaño, Jesus D. ; Zhang, Jiwei ; Schilling, Jonathan S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e876-5fcca6d6b6bcf7eb084a8569e1ace74c7e5503a24c3660d3031be9a8f15130823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amplex red 10</topic><topic>Brown rot</topic><topic>Fungi</topic><topic>Radical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castaño, Jesus D.</creatorcontrib><creatorcontrib>Zhang, Jiwei</creatorcontrib><creatorcontrib>Schilling, Jonathan S.</creatorcontrib><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castaño, Jesus D.</au><au>Zhang, Jiwei</au><au>Schilling, Jonathan S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition</atitle><jtitle>Journal of microbiological methods</jtitle><date>2018-02</date><risdate>2018</risdate><volume>145</volume><spage>10</spage><epage>13</epage><pages>10-13</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity.
•Hydrogen peroxide (H2O2) is a key oxidant produced by fungi decomposing wood.•Low H202 levels in decaying wood (in planta) evade detection via standard methods.•We compared H2O2 quantification in wood being decayed by a fungus Postia placenta.•In order of most sensitive to least Amplex Red10<ABTS<FOX<4-aminoantipyrine.•Amplex Red (LOQ=0.36μM) was best for measuring H2O2 (0.41–1.77μM) in planta.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.mimet.2017.12.004</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of microbiological methods, 2018-02, Vol.145, p.10-13 |
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| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | Amplex red 10 Brown rot Fungi Radical |
| title | Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition |
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