Evaluation of colorimetric assays for determination of H2O2in planta during fungal wood decomposition

Hydrogen peroxide (H2O2) plays a critical role in generating oxygen radicals as fungi attack and deconstruct plant cell walls. Its concentrations in planta, however, are often low during decomposition and evade detection by traditional methods. Here, we compared relevant methods and selected the best based on detection limits and selectivity. •Hydrogen peroxide (H2O2) is a key oxidant produced by fungi decomposing wood.•Low H202 levels in decaying wood (in planta) evade detection via standard methods.•We compared H2O2 quantification in wood being decayed by a fungus Postia placenta.•In order of most sensitive to least Amplex Red10