A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λ = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λ = 663...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2018, Vol.54 (3), p.299-302
Hauptverfasser: Ogasawara, Hiroaki, Grzybowski, Marek, Hosokawa, Riho, Sato, Yoshikatsu, Taki, Masayasu, Yamaguchi, Shigehiro
Format: Artikel
Sprache:eng
Schlagworte:
Calcium ions
Fluorescein
Fluorescent indicators
Scaffolds
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Zusammenfassung:The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λ = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λ = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc07344e