A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin

A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetica...

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Veröffentlicht in:	Analytical Sciences 2017/12/10, Vol.33(12), pp.1339-1344
Hauptverfasser:	NESELIOGLU, Salim, ERGIN, Merve, EREL, Ozcan
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetohydroxamic acid Analytical Chemistry Automation Catalysis Catalytic activity Ceruloplasmin Chemistry copper Correlation analysis Ferric ions Ferrous ions Ferroxidase Ions o-Dianisidine Oxidase Oxidation Phenylenediamine Spectrophotometry
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