A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin

A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin

A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetica...

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Veröffentlicht in:Analytical Sciences 2017/12/10, Vol.33(12), pp.1339-1344
Hauptverfasser: NESELIOGLU, Salim, ERGIN, Merve, EREL, Ozcan
Format: Artikel
Sprache:eng
Schlagworte:
Acetohydroxamic acid
Analytical Chemistry
Automation
Catalysis
Catalytic activity
Ceruloplasmin
Chemistry
copper
Correlation analysis
Ferric ions
Ferrous ions
Ferroxidase
Ions
o-Dianisidine
Oxidase
Oxidation
Phenylenediamine
Spectrophotometry
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Beschreibung
Zusammenfassung:A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.33.1339