A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin
A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetica...
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| Veröffentlicht in: | Analytical Sciences 2017/12/10, Vol.33(12), pp.1339-1344 |
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| container_title | Analytical Sciences |
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| creator | NESELIOGLU, Salim ERGIN, Merve EREL, Ozcan |
| description | A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p |
| doi_str_mv | 10.2116/analsci.33.1339 |
| format | Article |
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Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.33.1339</identifier><identifier>PMID: 29225221</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Acetohydroxamic acid ; Analytical Chemistry ; Automation ; Catalysis ; Catalytic activity ; Ceruloplasmin ; Chemistry ; copper ; Correlation analysis ; Ferric ions ; Ferrous ions ; Ferroxidase ; Ions ; o-Dianisidine ; Oxidase ; Oxidation ; Phenylenediamine ; Spectrophotometry</subject><ispartof>Analytical Sciences, 2017/12/10, Vol.33(12), pp.1339-1344</ispartof><rights>2017 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2017</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c661t-49f85be93d2110f71a7967f1ebcc1a94d999135cffd6ed9f5ebed2a9d1e3dec3</citedby><cites>FETCH-LOGICAL-c661t-49f85be93d2110f71a7967f1ebcc1a94d999135cffd6ed9f5ebed2a9d1e3dec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.33.1339$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.33.1339$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,1881,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29225221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NESELIOGLU, Salim</creatorcontrib><creatorcontrib>ERGIN, Merve</creatorcontrib><creatorcontrib>EREL, Ozcan</creatorcontrib><title>A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.</description><subject>Acetohydroxamic acid</subject><subject>Analytical Chemistry</subject><subject>Automation</subject><subject>Catalysis</subject><subject>Catalytic activity</subject><subject>Ceruloplasmin</subject><subject>Chemistry</subject><subject>copper</subject><subject>Correlation analysis</subject><subject>Ferric ions</subject><subject>Ferrous ions</subject><subject>Ferroxidase</subject><subject>Ions</subject><subject>o-Dianisidine</subject><subject>Oxidase</subject><subject>Oxidation</subject><subject>Phenylenediamine</subject><subject>Spectrophotometry</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kUFvEzEQRi1ERUPhzA1Z4sKBTT32rhMfV4ECagWXcrYce9xutLsOthfIv8dR0qBW4uTDvO-z55mQN8DmHEBemtH0yXZzIeYghHpGZiDqZcV5LZ-TGVPAKilqdk5eprRhDPiS8xfknCvOG85hRn609Bv-ptfdiLmzH2g75TCYjI62KZkdzYF-xIxxKADN90ivMMbwp3MmIW1t7n51eUeDpyuMUx-2vUkFfUXOfHkYvj6eF-T26tPt6kt18_3z11V7U1kpIVe18stmjUq4sgzzCzALJRcecG0tGFU7pRSIxnrvJDrlG1yj40Y5QOHQigvy_lC7jeHnhCnroUsW-96MGKakQS2aRtWylgV99wTdhCnu7WnOuQLZyLop1OWBsjGkFNHrbewGE3camN4L10fhWgi9F14Sb4-903pAd-IfDBeAHYBURuMdxn8X_79zdYhsUjZ3eOo0sXxRj494_pA6Te29iRpH8Rcf0KY0</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>NESELIOGLU, Salim</creator><creator>ERGIN, Merve</creator><creator>EREL, Ozcan</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin</title><author>NESELIOGLU, Salim ; ERGIN, Merve ; EREL, Ozcan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c661t-49f85be93d2110f71a7967f1ebcc1a94d999135cffd6ed9f5ebed2a9d1e3dec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acetohydroxamic acid</topic><topic>Analytical Chemistry</topic><topic>Automation</topic><topic>Catalysis</topic><topic>Catalytic activity</topic><topic>Ceruloplasmin</topic><topic>Chemistry</topic><topic>copper</topic><topic>Correlation analysis</topic><topic>Ferric ions</topic><topic>Ferrous ions</topic><topic>Ferroxidase</topic><topic>Ions</topic><topic>o-Dianisidine</topic><topic>Oxidase</topic><topic>Oxidation</topic><topic>Phenylenediamine</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NESELIOGLU, Salim</creatorcontrib><creatorcontrib>ERGIN, Merve</creatorcontrib><creatorcontrib>EREL, Ozcan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NESELIOGLU, Salim</au><au>ERGIN, Merve</au><au>EREL, Ozcan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. 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This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.</abstract><cop>Singapore</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>29225221</pmid><doi>10.2116/analsci.33.1339</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetohydroxamic acid Analytical Chemistry Automation Catalysis Catalytic activity Ceruloplasmin Chemistry copper Correlation analysis Ferric ions Ferrous ions Ferroxidase Ions o-Dianisidine Oxidase Oxidation Phenylenediamine Spectrophotometry |
| title | A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin |
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