Purification and activity characterization of polysaccharides in the medicinal lichen Umbilicaria tornata from Taibai Mountain, China
Water-soluble polysaccharides from Umbilicaria tornata (UTP) were purified and preliminarily characterized. The antioxidant and antitumor activities of crude UTP and two purified fractions (UTP-1 and UTP-2) were evaluated using in vitro experiments. The results showed that the molecular weights of U...
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Veröffentlicht in: | Glycoconjugate journal 2018-02, Vol.35 (1), p.107-117 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Water-soluble polysaccharides from
Umbilicaria tornata
(UTP) were purified and preliminarily characterized. The antioxidant and antitumor activities of crude UTP and two purified fractions (UTP-1 and UTP-2) were evaluated using
in vitro
experiments. The results showed that the molecular weights of UTP-1 and UTP-2 were 84.86 and 28.66 kDa, respectively. Both UTP-1 and UTP-2 were composed of glucose and xylose, with their molar ratios being 1.3:0.9 and 0.9:4.6, respectively. In addition, crude UTP, UTP-1 and UTP-2 showed dose-dependent DPPH and hydroxyl radical scavenging and reducing activities. However, crude UTP exhibited stronger antioxidant activity than UTP-1 and UTP-2, particularly in terms of DPPH radicals. Crude UTP and the two purified fractions inhibited the growth of HeLa, HepG2, A375, MCF-7, SGC7901 and Caco2 cancer cells
in vitro
. Compared with UTP-1 and UTP-2, crude UTP presented significantly higher antitumor activity
in vitro
against HeLa and HepG2 cells (
p
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ISSN: | 0282-0080 1573-4986 |
DOI: | 10.1007/s10719-017-9806-3 |