Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 6...
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| Veröffentlicht in: | Nature methods 2007-08, Vol.4 (8), p.641-643 |
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| creator | Gambhir, Sanjiv Sam Loening, Andreas Markus Wu, Anna M |
| description | The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc. |
| doi_str_mv | 10.1038/nmeth1070 |
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To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth1070</identifier><identifier>PMID: 17618292</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Animals ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; brief-communication ; Color ; Emission spectroscopy ; Genetic variation ; Genetics ; Humans ; Life Sciences ; Light ; Luciferase ; Luciferases, Renilla - genetics ; Luciferases, Renilla - metabolism ; Luminescence ; Marine ; Mice ; Mutagenesis, Site-Directed ; Proteomics ; Renilla - enzymology ; Renilla reniformis ; Research methodology ; Scientific imaging ; Wavelengths</subject><ispartof>Nature methods, 2007-08, Vol.4 (8), p.641-643</ispartof><rights>Springer Nature America, Inc. 2007</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Aug 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-631faa2605cf8e6bc700973181b96cef54f0fabf29a62f1f45d2c020ba0a4f0e3</citedby><cites>FETCH-LOGICAL-c467t-631faa2605cf8e6bc700973181b96cef54f0fabf29a62f1f45d2c020ba0a4f0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth1070$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth1070$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2725,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17618292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gambhir, Sanjiv Sam</creatorcontrib><creatorcontrib>Loening, Andreas Markus</creatorcontrib><creatorcontrib>Wu, Anna M</creatorcontrib><title>Red-shifted Renilla reniformis luciferase variants for imaging in living subjects</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.</description><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>brief-communication</subject><subject>Color</subject><subject>Emission spectroscopy</subject><subject>Genetic variation</subject><subject>Genetics</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Luciferase</subject><subject>Luciferases, Renilla - genetics</subject><subject>Luciferases, Renilla - metabolism</subject><subject>Luminescence</subject><subject>Marine</subject><subject>Mice</subject><subject>Mutagenesis, Site-Directed</subject><subject>Proteomics</subject><subject>Renilla - 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Renilla reniformis luciferase variants for imaging in living subjects</title><author>Gambhir, Sanjiv Sam ; Loening, Andreas Markus ; Wu, Anna M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-631faa2605cf8e6bc700973181b96cef54f0fabf29a62f1f45d2c020ba0a4f0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biological Microscopy</topic><topic>Biological Techniques</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>brief-communication</topic><topic>Color</topic><topic>Emission spectroscopy</topic><topic>Genetic variation</topic><topic>Genetics</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Luciferase</topic><topic>Luciferases, Renilla - genetics</topic><topic>Luciferases, Renilla - 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| subjects | Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Color Emission spectroscopy Genetic variation Genetics Humans Life Sciences Light Luciferase Luciferases, Renilla - genetics Luciferases, Renilla - metabolism Luminescence Marine Mice Mutagenesis, Site-Directed Proteomics Renilla - enzymology Renilla reniformis Research methodology Scientific imaging Wavelengths |
| title | Red-shifted Renilla reniformis luciferase variants for imaging in living subjects |
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