Red-shifted Renilla reniformis luciferase variants for imaging in living subjects

The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 6...

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Veröffentlicht in:	Nature methods 2007-08, Vol.4 (8), p.641-643
Hauptverfasser:	Gambhir, Sanjiv Sam, Loening, Andreas Markus, Wu, Anna M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Color Emission spectroscopy Genetic variation Genetics Humans Life Sciences Light Luciferase Luciferases, Renilla - genetics Luciferases, Renilla - metabolism Luminescence Marine Mice Mutagenesis, Site-Directed Proteomics Renilla - enzymology Renilla reniformis Research methodology Scientific imaging Wavelengths
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Beschreibung
Zusammenfassung:	The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth1070