Bond strength of adhesive systems to sound and demineralized dentin treated with bioactive glass ceramic suspension

Objective The objective of this study was to evaluate the effect of a Biosilicate®, associated with dentin adhesive system, on microtensile bond strength (μTBS) to sound and demineralized dentin. Materials and methods Eighty sound-extracted molars had their middle occlusal dentin exposed. In forty teeth, dentin was artificially demineralized (pH cycling). Sound and demineralized dentin teeth were separated into four groups ( n = 10), according to the substrate treatment before restoration: Group 1—total-etching adhesive Adper TM Single bond 2 (ASB) + Biosilicate®, Group 2—ASB (without Biosilicate®), Group 3—AdheSE self-etching adhesive system (AdSE) + Biosilicate®, and Group 4—AdSE (without Biosilicate®). Each tooth was restored with a hybrid composite and stored in water at 37 °C for 6 months. After water aging, teeth were cut in sticks (≈ 1 mm 2 cross-sectional area) and all samples were submitted to μTBS test. The fracture modes of the samples were analyzed by stereomicroscopy. The representatively fractured samples were observed by scanning electron microscopy . Representative samples of each group were analyzed on energy dispersive X-ray spectrometry (EDX). The μTBS and Ca-P ratio values were analyzed by 2-way ANOVA, Bonferroni, and Tukey test, respectively, p