Visualization of dendritic cells’ responses in atopic dermatitis: Preventing effect of emollient

Atopic dermatitis (AD) is a chronic and multifactorial inflammatory skin disease involving various dendritic cells such as epidermal Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDECs). Most of the clinical studies was performed on isolated cells, and thus, it would be useful to...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Experimental dermatology 2018-04, Vol.27 (4), p.374-377
Hauptverfasser: Mias, Céline, Le Digabel, Jimmy, Filiol, Jérôme, Gontier, Etienne, Gravier, Eléonore, Villaret, Aurélie, Nocera, Thérèse, Questel, Emmanuel, Rossi, Ana‐Beatris, Redoulès, Daniel, Josse, Gwendal
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Atopic dermatitis (AD) is a chronic and multifactorial inflammatory skin disease involving various dendritic cells such as epidermal Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDECs). Most of the clinical studies was performed on isolated cells, and thus, it would be useful to characterize directly on the human epidermal tissue the first cellular events occurred during the AD. The suction blister method was used to obtain whole epidermis samples and interstitial cutaneous fluids. Employing multiphoton microscopy, we analyzed the early dynamic behavior of inflammatory cells using Dermatophagoides pteronyssinus atopy patch test (Derp‐APT) and evaluated the effects of emollient pre‐application. Derp‐APT application provoked rapid and strong infiltration of IDECs, and proliferation and activation of LC in the AD subjects’ epidermis. Moreover, emollient pre‐application strengthened the defective skin barrier and had positive effects on inflammatory cells’ behavior, characterized by the complete inhibition of IDEC influx and the presence of immature LC.
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.13470