A colorimetric competitive displacement assay for the evaluation of catalytic peptides

A colorimetric competitive displacement assay for the evaluation of catalytic peptides

An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is dem...

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Veröffentlicht in:Organic & biomolecular chemistry 2017, Vol.15 (48), p.10160-10163
Hauptverfasser: Gest, Anneliese M M, Aguiluz, Erika M, Mays, Malik T, Liu, Xinyu, Neidhart, Eliza K, Witus, Leah S
Format: Artikel
Sprache:eng
Schlagworte:
Aldehydes
Catalysis
Catalysts
Colorimetry
Peptides
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Zusammenfassung:An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.
ISSN:1477-0520
1477-0539
DOI:10.1039/c7ob02032e