Metabolites from the marine fungus Eurotium repens

1,8-Dihydroxy-6-methoxy-3-methyl-9,10-anthracenedione (physcion, 1), 3,4-dihydro-3,6,9-trihydroxy-8-methoxy-3-methyl-1(2H)-anthraceneon e (asperflavin, 2), and 2,5-dihydroxy-3-(3-methyl-2-butenyl)-6-[(1E)-1-heptenyl]-benzal dehyde (tetrahydroauroglaucin, 3) were shown to be the main pigments of the marine isolate of the fungus Eurotium repens. In addition to the pigments, the fungal metabolites included the diketopiperazine alkaloid echinulin (4). The structures of the compounds were identified using NMR spectroscopy and mass spectrometry. The cytotoxic activity of 1-3 toward sex cells of the sea urchin Strongylocentrotus intermedius was determined.