Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N6-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Fu...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2017-12, Vol.53 (96), p.12930-12933
Hauptverfasser: Imanishi, Miki, Tsuji, Shogo, Suda, Akiyo, Futaki, Shiroh
Format: Artikel
Sprache:eng
Schlagworte:
E coli
Ribonucleic acid
RNA
Sensitivity analysis
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Zusammenfassung:We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N6-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc07699a