Suitability of a chocolate agar-based medium for the transportation and long term storage of Neisseria gonorrhoeae isolates at room temperature

Neisseria gonorrhoeae isolates were grown on chocolate agar slants comprising GC Agar Medium Base supplemented with 1% modified Kellogg's supplement and 1% beef blood hemoglobin. The bacterial growth was covered with sterilized paraffin oil. Cultures were used for transportation and storage of Neisseria gonorrhoeae for 3months at room temperature. •A transport and storage medium for N. gonorrhoeae was evaluated.•The medium comprises supplemented chocolate agar covered with sterile paraffin.•Isolates can survive at room temperature for at least 10weeks.