Epigenetic silencing of ADAMTS5 is associated with increased invasiveness and poor survival in patients with colorectal cancer

Purpose A disintegrin and metalloprotease with motif 5(ADAMTS5) has been involved in colorectal cancer (CRC) with hypermethylation in the promoter. However, its role in CRC remains unclear. The aim of this study was to explore the clinical significance and biological effect of ADAMTS5 on colorectal...

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Veröffentlicht in:	Journal of cancer research and clinical oncology 2018-02, Vol.144 (2), p.215-227
Hauptverfasser:	Li, Jizhen, Liao, Yi, Huang, Jintuan, Sun, Yi, Chen, Hao, Chen, Chunyu, Li, Senmao, Yang, Zuli
Format:	Artikel
Sprache:	eng
Schlagworte:	ADAMTS5 Protein - biosynthesis ADAMTS5 Protein - genetics Apoptosis Base Sequence Biomarkers, Tumor Cancer Research Carcinogenesis Cell cycle Cell Line, Tumor Cell Movement - genetics Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology CpG Islands Disease-Free Survival DNA Methylation Epigenesis, Genetic Female Gene Silencing HCT116 Cells Hematology HT29 Cells Humans Internal Medicine Invasiveness Male Medicine Medicine & Public Health Metalloproteinase Middle Aged Neoplasm Invasiveness Oncology Original Article – Cancer Research Promoter Regions, Genetic Survival Rate
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Zusammenfassung:	Purpose A disintegrin and metalloprotease with motif 5(ADAMTS5) has been involved in colorectal cancer (CRC) with hypermethylation in the promoter. However, its role in CRC remains unclear. The aim of this study was to explore the clinical significance and biological effect of ADAMTS5 on colorectal carcinogenesis. Through MSP, qRT-PCR, WB and IHC analysis, followed by a variety of in vitro assays, we report the function of ADAMTS5 in CRC. ADAMTS5 was markedly hypermethylaed and downregulated in tumor tissues compared with non-tumor tissues ( p
ISSN:	0171-5216 1432-1335
DOI:	10.1007/s00432-017-2545-9