Aidingibacillus halophilus gen. nov., sp. nov., a novel member of the family Bacillaceae

A Gram-positive, non-motile, asporogenous and aerobic bacterium, designated YIM 98012 T , was isolated from a salt lake in China. Strain YIM 98012 T was found to be catalase and oxidase positive. Optimal growth of strain YIM 98012 T was observed at 37 °C and pH 7.0 and it was found to grow in the presence of 5–20% (w/v) NaCl (optimum 10% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel strain is affiliated with the family Bacillaceae of the phylum Firmicutes and that it shares high (94.7%) sequence similarity with Alteribacillus persepolensis DSM 21632 T and does not show sequence similarities of more than 94.0% to known members of other related genera. The major fatty acids (> 10%) were identified as anteiso-C 15:0 , anteiso-C 17:0 , iso-C 16:0 and C 16:0 . The genomic DNA G+C content was determined to be 41.0 mol% and the dominated respiratory quinone was identified as MK-7. The cell wall peptidoglycan of strain YIM 98012 T was found to contain meso -diaminopimelic acid, while the polar lipids profile was found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. Based on physiological and chemotaxonomic characteristics, strain YIM 98012 T is concluded to be the type strain of the type species of a novel genus in the family Bacillaceae for which the name Aidingibacillus halophilus gen. nov., sp. nov. is proposed. The type strain is YIM 98012 T (= KCTC 33868 T = DSM 104332 T ).