Maternal methyl donor supplementation during gestation counteracts bisphenol A-induced oxidative stress in sows and offspring

Abstract Objective Maternal oxidative stress is harmful for embryonic, fetal and placental development. This experiment was conducted to investigate whether methyl donor supplementation during gestation effectively ameliorates maternal and placenta oxidative stress up to offspring. Methods Fifty-six Landrace × Yorkshire sows were randomly allocated to receive one of the following four diets during gestation: control diet (CON); control diet supplemented with methyl donor (MET); control diet supplemented with bisphenol-A (BPA); and control diet supplemented with BPA and MET (BPA + MET). Blood sample, chorioallantois and piglets liver samples were analyzed for antioxidant status and mRNA expression of genes in regard to oxidative status. Results MET diets lowered homocysteine concentration in sows plasma, improved activity of antioxidant enzymes in chorioallantois and piglets plasma, liver (catalase, glutathione peroxidase and total antioxidant capacity, p < 0.01), as also upregulated mRNA expression of Cu,Zn superoxide dismutase (p < 0.01) and glutathione peroxidase 1 (p < 0.05) in the chorioallantois and CAT (p < 0.01) in piglets liver compared with control group. In contrast, BPA diets increased malondialdehyde (p < 0.01) level in sows and piglets and decreased T-AOC (p < 0.01) concentration in sows and umbilical cord blood plasma, as also down-regulated Cu,Zn-SOD (p = 0.01) in piglets liver compared with MET group. Conclusion BPA diets fed to sows during gestation aggravated oxidative stress status in sows and piglets, while the methyl donor diets enhanced antioxidant capacity of sows and piglets and ameliorated oxidative stress induced by BPA.