Rapid Diagnosis of Highly Pathogenic Avian Influenza Using Pancreatic Impression Smears
The 1985 outbreak of high-pathogenicity avian influenza (HPAI) in Victoria, Australia, took 5 days to confirm by standard laboratory tests, during which time infected chickens continued excreting virus, thus creating the opportunity for transmission to other farms. An immunofluorescence test for the...
Gespeichert in:
Veröffentlicht in: | Avian diseases 2003-09, Vol.47 (s3), p.1190-1195 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The 1985 outbreak of high-pathogenicity avian influenza (HPAI) in Victoria, Australia, took 5 days to confirm by standard laboratory tests, during which time infected chickens continued excreting virus, thus creating the opportunity for transmission to other farms. An immunofluorescence test for the detection of viral antigen in tissue impression smears was evaluated as a rapid diagnostic test for HPAI virus infections of poultry. Several test configurations were compared for background reactions and strength of fluorescence, with the optimum combination found to be an influenza A group-specific monoclonal antibody, detected by an anti-mouse fluorescein isothiocyanate conjugate. Immunohistochemical examination of tissues from chickens experimentally infected with low-pathogenicity and HPAI viruses identified the pancreas as the organ most consistently containing high concentrations of HPAI viral antigen. This test has since been used in Australia in the rapid laboratory confirmation of three avian influenza outbreaks and in showing that numerous other suspect cases were not caused by avian influenza.Original Abstract: Diagnostico rapido de virus de influenza aviar altamente patogeno mediante frotis de pancreas.Durante el brote de influenza aviar de alta patogenicidad en 1985 en Victoria, Australia, se requirieron 5 dias para confirmar el diagnostico por medio de pruebas estandar de laboratorio. Durante ese periodo las aves infectadas continuaron excretando virus, aumentando la posibilidad de transmision del virus a otras granjas. Una prueba de inmunofluorescencia para la deteccion de antigeno viral en frotis de tejidos fue evaluada como una prueba de diagnostico rapido para las infecciones por virus de influenza aviar de alta patogenicidad en la avicultura. Varias configuraciones de la prueba fueron comparadas con relacion a la tincion de fondo inespecifica y a la intensidad de la fluorescencia. La combinacion optima se encontro utilizando un anticuerpo monoclonal especifico de grupo para el virus de influenza aviar grupo A, detectado por un anticuerpo anti-raton conjugado con isotiocianato de fluoresceina. Al examen por inmunohistoquimica de tejidos de pollos infectados experimentalmente con virus de influenza de baja o de alta patogenicidad, se identifico al pancreas como el organo que contenia las mas altas concentraciones del antigeno contra el virus de influenza de alta patogenicidad. Esta prueba ha sido usada desde entonces en Australia para la confi |
---|---|
ISSN: | 0005-2086 |
DOI: | 10.1043/0005-2086(2003)047<1190:RDOHPA>2.0.CO;2 |