Psedoduganella eburnea sp. nov., isolated from lagoon sediments

Strain 10R5-21 was isolated from lagoon sediments. Cells of strain 10R5-21 were Gram-reaction-negative, rod-shaped and motile by means of polar flagella. The strain was obligately aerobic and positive for catalase and oxidase activity. Strain 10R5-21 was able to grow at 10-37 ˚C (optimum 25-30 ˚C), at pH 5.0-9.0 (optimum pH 6.5-7.5) and in the presence of 0-0.5 % (w/v) NaCl (optimum 0 %). C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C12 : 0 were present as predominant (>5 %) fatty acids. Q-8 was identified as the major respiratory quinone. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were present as major polar lipids with minor amounts of unidentified aminophospholipids and unidentified aminolipids. The genomic G+C content of strain 10R5-21 was 64.1 mol%. 16S rRNA gene sequence analysis indicated that strain 10R5-21 belongs to the genus Pseudoduganella within the family Oxalobacteraceae of the class Betaproteobacteria. Strain 10R5-21 shared 98.8 % 16S rRNA gene sequence similarity with Pseudoduganella violaceinigra YIM 31327 . DNA-DNA hybridization values between strain 10R5-21 and P. violaceinigra KACC 11669 were clearly below the 70 % threshold. Distinct morphological, biochemical, chemotaxonomic and genetic differences from previously described taxa support the classification of strain 10R5-21 as a representative of a novel species in the genus Pseudoduganella, for which the name Pseudoduganella eburnea sp. nov. is proposed. The type strain is 10R5-21 (=KEMB 563-061 =JCM 31587 ).