Analysis of the global regulator Lae1 uncovers a connection between Lae1 and the histone acetyltransferase HAT1 in Fusarium fujikuroi
The fungus Fusarium fujikuroi causes “ bakanae ” disease of rice due to its ability to produce gibberellins (GAs), a family of plant hormones. Recent genome sequencing revealed the genetic capacity for the biosynthesis of 46 additional secondary metabolites besides the industrially produced GAs. Amo...
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Veröffentlicht in: | Applied microbiology and biotechnology 2018, Vol.102 (1), p.279-295 |
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Zusammenfassung: | The fungus
Fusarium fujikuroi
causes “
bakanae
” disease of rice due to its ability to produce gibberellins (GAs), a family of plant hormones. Recent genome sequencing revealed the genetic capacity for the biosynthesis of 46 additional secondary metabolites besides the industrially produced GAs. Among them are the pigments bikaverin and fusarubins, as well as mycotoxins, such as fumonisins, fusarin C, beauvericin, and fusaric acid. However, half of the potential secondary metabolite gene clusters are silent. In recent years, it has been shown that the fungal specific
velvet
complex is involved in global regulation of secondary metabolism in several filamentous fungi. We have previously shown that deletion of the three components of the
F. fujikuroi velvet
complex,
vel1
,
vel2
, and
lae1
, almost totally abolished biosynthesis of GAs, fumonisins and fusarin C. Here, we present a deeper insight into the genome-wide regulatory impact of Lae1 on secondary metabolism. Over-expression of
lae1
resulted in de-repression of GA biosynthetic genes under otherwise repressing high nitrogen conditions demonstrating that the nitrogen repression is overcome. In addition, over-expression of one of five tested histone acetyltransferase genes,
HAT1
, was capable of returning GA gene expression and GA production to the GA-deficient Δ
lae1
mutant. Deletion and over-expression of
HAT1
in the wild type resulted in downregulation and upregulation of GA gene expression, respectively, indicating that HAT1 together with Lae1 plays an essential role in the regulation of GA biosynthesis. |
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ISSN: | 0175-7598 1432-0614 |
DOI: | 10.1007/s00253-017-8590-0 |