Plant Transformation: Needs and Futurity of the Transgenes

To produce transgenic plants which have various applications in agricultural and non-agricultural fields, a marker gene is necessary to recover a viable transgenic plant. To express or transcribe of transgenes, utilization of promoters is also unavoidable. Analysis of transgenes includes copy number...

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Veröffentlicht in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2008-09, Vol.7 (3), p.403-412
Hauptverfasser: Darbani, Behrooz, Farajnia, Safar, Noeparvar, Shahin, Neal Stewa, C., Mohammadi, Seyed A., Zakerbosta, Saeed
Format: Artikel
Sprache:eng
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Zusammenfassung:To produce transgenic plants which have various applications in agricultural and non-agricultural fields, a marker gene is necessary to recover a viable transgenic plant. To express or transcribe of transgenes, utilization of promoters is also unavoidable. Analysis of transgenes includes copy number, insertion site, integration stability, expression and it's pattern and variability is immensely important in order to develop a successful transgenic event. This review presents the necessities for better recovery of transgenic plants, transcription or expression of transgenes, as well as methods to analyze transgenes.
ISSN:1682-296X
DOI:10.3923/biotech.2008.403.412