Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein—Barr Virus Latent Phase Protein LMP1

Intracellular fragments of latent phase protein LMP1 of Epstein—Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the transforming potential of the virus. Generation of recombinant proteins CTAR1/2/3 is expected to yield more ample data on functional and immu...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2017-10, Vol.163 (6), p.766-771
Hauptverfasser:	Lomakin, Ya. A., Shmidt, A. A., Bobik, T. V., Chernov, A. S., Pyrkov, A. Yu, Aleksandrova, N. M., Okunola, D. O., Vaskina, M. I., Ponomarenko, N. A., Telegin, G. B., Dubina, M. V., Belogurov, A. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Antibodies, Viral - biosynthesis Antibodies, Viral - chemistry Antibodies, Viral - isolation & purification Antigenic determinants B cells Biomedical and Life Sciences Biomedicine Cell Biology Cloning, Molecular Epitope Mapping - methods Epitopes - genetics Epitopes - immunology Epstein-Barr virus Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genetic Vectors - chemistry Genetic Vectors - metabolism Herpesvirus 4, Human - chemistry Herpesvirus 4, Human - genetics Herpesvirus 4, Human - immunology Humans Immune response Immunization Internal Medicine Laboratory Medicine Mice Monoclonal antibodies Pathology Peptide Fragments - genetics Peptide Fragments - immunology Peptide Library Protein Domains Recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - immunology Viral Matrix Proteins - genetics Viral Matrix Proteins - immunology Virus Latency - immunology
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Zusammenfassung:	Intracellular fragments of latent phase protein LMP1 of Epstein—Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the transforming potential of the virus. Generation of recombinant proteins CTAR1/2/3 is expected to yield more ample data on functional and immunogenic characteristics of LMP1. We created genetic constructs for prokaryotic expression of LMP1 CTAR fragments and selected optimal conditions for their production and purification. Using a new library of LMP1 CTAR fragments, we carried out epitope mapping of a diagnostic anti-LMP1 antibody S12. Analysis of polyclonal serum antibodies from mice immunized with full-length LMP1 confirmed immunogenicity of CTAR elements comparable with that of full-length protein
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-017-3899-3