Promotion of metal accumulation in nodule of Astragalus sinicus by the expression of the iron-regulated transporter gene in Mesorhizobium huakuii subsp. rengei B3
Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between Astragalus sinicus and the recombinant rhizobium, Mesorhizobium huakuii subsp. rengei B3 developed by overexpressing a syntheti...
Gespeichert in:
Veröffentlicht in: | Journal of bioscience and bioengineering 2008-06, Vol.105 (6), p.642-648 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Toxic metal contamination in agricultural fields is an important worldwide problem. In previous studies, we developed a bioremediation system based on the symbiosis between
Astragalus sinicus and the recombinant rhizobium,
Mesorhizobium huakuii subsp.
rengei B3 developed by overexpressing a synthetic tetrameric metallothionein gene (
MTL4) and cDNA encoding the phytochelatin synthase from
Arabidopsis thaliana (
AtPCS). To promote the transport of metals into the nodules of the rhizobium and the accumulation of metals, the iron-regulated transporter 1 gene from
A. thaliana (
AtIRT1) was introduced into recombinant strain B3 containing
MTL4 or
AtPCS in its chromosome. The fused AtIRT1-alkaline phosphatase was expressed in the free-living recombinant rhizobium and the nodule of
A. sinicus. The recombinant strain B3 carrying
AtIRT1 showed a higher Cd sensitivity and a higher amount of Cd accumulated in free-living culture than the wild-type strain B3. When the recombinant strain B3 established symbiosis with
A. sinicus, the introduction of
AtIRT1 in the recombinant strain B3 advantaged the accumulation of Cu and As in the nodules of
A. sinicus, compared with that of Cd and Zn. |
---|---|
ISSN: | 1389-1723 1347-4421 |
DOI: | 10.1263/jbb.105.642 |