Obtaining transgenic bovine skin fibroblasts containing human interferon alpha gene

The study aimed at obtaining the heifer's transgenic cell lines with integrated human interferon alpha gene (IFN alpha ) having in view cloning of Polish Red cattle. The gene construct used (pbLGIFN-GFPBsd) contained the entire human interferon alpha gene under the bovine beta -lactoglobulin promoter (bLG) and the green fluorescent protein gene (gfp) fused to the blasticidin resistance gene (bsd) under the human cytomegalovirus (CMV) immediate-early promoter. The gene construct was introduced by lipofection into heifer's ear fibroblasts. The GFP- and Bsd-positive cell lines have been obtained. The molecular analysis (PCR) and cytogenetic characterization (FISH) confirmed obtaining the stable heifer's transgenic cell lines with integrated human IFN alpha gene.